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Reagent Lab Preparation For DNA Isolation

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Reagent for DNA Isolation

  • CTAB/NaCl solution (10% CTAB, 0.7 M NaC1)

Dissolve 10 g of CTAB in 80 mL of 0.7 M NaCl solution. Stir on a hot magnetic  stirrer until the CTAB has dissolved. Adjust volume to 100 mL with 0.7 M NaCl solution. Read more »

Reagent Lab Preparation For Biochemistry Practice

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REAGENT LAB PREPARATION

1.Anthrone Reagent

Dissolve 2 g of anthrone in 1 liter of concentrated sulfuric acid. Store in a refrigerator. Prepare a fresh solution every 2 day

2. Benedict’s Qualitative Solution

Dissolve 173 g of sodium citrate dehydrate and 100 g sodium carbonate anhydrous in 800 mL DI water. Warm and stir to aid dissolution. Filter if necessary. In a separate container, dissolve 17.3 g copper (II) sulfate pentahydrate in 100 mL DI water. Slowly, while stirring constantly, add the copper sulfate solution to the first solution. Let cool and dilute to 1 L with DI water. (test for the presence of simple sugars)

3. Iodine Solution, Lugol’s

Dissolve 20 g of potassium iodide in 200 mL of DI water; add 10 g of iodine; stir to dissolve then dilute to 1 L. Store in a dark bottle. (general biological stain and vital stain stock solution, dilute 5:1 before use.) Read more »

Chemistry Ebook: Recommended Methods of Manure Analysis

manure analysis

Unit I
Sampling Livestock Waste for Analysis
1. Introduction
2. Sampling livestock waste
2.1 Technique
2.2 Time
2.3 Storage management
3. Sampling recommendations
4.Recommended procedures for sampling livestock waste
4.1 Solid manure dairy, beef, swine, poultry
4.2 Liquid manure dairy, beef, swine
4.3 Sample identification and delivery
5. References

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