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Prosedur Penggunaan Plant Preservative Mixture (PPM) Pada Media Kultur Jaringan Untuk Peningkatan Kualitas dan Kuantitas Tanaman Kultur Jaringan

Tanaman Kultur Jaringan dengan Plant Preservative Mixture Kultur jaringan tanaman (Picture from http://www.scind.org)

Kultur jaringan dikenal juga dengan sebutan tissue culture. Kultur jaringan berasal dari kata kultur  yang artinya budidaya dan jaringan yang artinya sekelompok sel yg mempunyai bentuk dan fungsi yang sama. Berdasarkan asal kata tersebut maka arti dari kultur jaringan adalah membudidayakan jaringan tanaman menjadi tanaman baru yang mempunyai sifat sama dengan induknya. Kultur Jaringan diartikan pula dengan memelihara & menumbuhkan organ tanaman (embrio, tunas, bunga dsb) atau jaringan tanaman (sel, kalus, protoplast) pada kondisi aseptik. (https://www.pintarbiologi.com). Salah satu cara untuk peningkatan kualitas dan kuantitas tanaman kultur jaringan yaitu dengan penggunaan Plant Preservative Mixture (PPM). Plant Preservative Mixture (PPM) merupakan preservative/biosida spectrum luas yang efektif untuk mencegah atau menurunkan kontaminasi mikroba pada kultur jaringan tanaman. Pada dosis optimum PPM sangat efektif dan tidak mempengaruhi vitro germination, ploriferasi kalus dan regenerasi kalus. Plant Preservative Mixture (PPM) ini mengandung zat aktif yang terdiri dari :

5-Chloro-2 methyl-3-(CH) – isothiazolone 0,1350%
2-methyl-3(H)-isothiazolone 0,0412%

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Biology Ebook: Practical Handbook of Microbiology Second Edition Edited By Emanuel Goldman and Lorrence H. Green

Handbook of MicrobiologyThe first edition of the Practical Handbook of Microbiology was published in 1989. Since that time, the field of microbiology has undergone many changes and has grown to encompass other disciplines as well. New chapters have been added and a number of chapters from the first edition were dropped. Tables in the first edition that were outdated have been replaced by tables in the individual chapters. This edition also contains a new broad and concise survey table of selected eubacteria. Areas generally considered part of microbiology that were not covered or covered only briefly in the first edition are now included with comprehensive introductory chapters. This book was written to provide basic knowledge and practical information about working with microorganisms, in a clear and concise form. Although of use to anyone interested in the subject matter, the book is intended to especially benefit two groups of microbiologists: people trained as microbiologists who are highly specialized in working with one specific area of microbiology; and people who have been trained in other disciplines and use microorganisms as simply another tool or chemical reagent. Read more »

Microbilogy Ebook: Handbook of Microbiological Media Fourth Edition By Ronald M. Atlas

handbook-of-microbiological-media-fourth-edition-2010-ronald-m-atlaS(Picture from https://image.slidesharecdn.com)

Handbook of Microbiological Media Fourth Edition By Ronald M. Atlas includes the formulations and descriptions of 7,080 media used for cultivating microorganisms more than in the previous edition. These include both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi. Some of these microbiological media are produced by major suppliers of dehydrated media including Oxoid, HiMedia, and BD Diagnostics (Difco, BBL, and GIBCO). These include all the media normally used in the clinical microbiology diagnostic laboratory and for the routine examination of food and water. Other media described in the fourth edition of the Handbook of Microbiological Media are used to cultivate specific strains of bacteria, archaea, fungi, and protists, including many anaerobes and extremophiles. Read more »

Biology Ebook: Laboratory Excercise In Microbiology By Prescott Harley 5th Edition

laboratory excercise

Prescott, Harley and Klein’s 5th edition provides a balanced, comprehensive introduction to all major areas of microbiology. Because of this balance, Microbiology, 5/e is appropriate for students preparing for careers in medicine, dentistry, nursing, and allied health, as well as research, teaching, and industry. Biology and chemistry are prerequisites.

Brief content of Laboratory Excercise in Microbiology:

Laboratory Skills
A student successfully completing basic microbiology will demonstrate the ability to
1. Use a bright-field light microscope to view and interpret slides, including
a. correctly setting up and focusing the microscope
b. proper handling, cleaning and storage of the microscope
c. correct use of all lenses
d. recording microscopic observations
2. Properly prepare slides for microbiological examination, including
a. cleaning and disposal of slides
b. preparing smears from solid and liquid cultures
c. performing wet-mount and/or hanging drop preparations
d. performing Gram stains Read more »