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Reagent Lab Preparation For Biochemistry Practice

lab regaent

REAGENT LAB PREPARATION

1.Anthrone Reagent

Dissolve 2 g of anthrone in 1 liter of concentrated sulfuric acid. Store in a refrigerator. Prepare a fresh solution every 2 day

2. Benedict’s Qualitative Solution

Dissolve 173 g of sodium citrate dehydrate and 100 g sodium carbonate anhydrous in 800 mL DI water. Warm and stir to aid dissolution. Filter if necessary. In a separate container, dissolve 17.3 g copper (II) sulfate pentahydrate in 100 mL DI water. Slowly, while stirring constantly, add the copper sulfate solution to the first solution. Let cool and dilute to 1 L with DI water. (test for the presence of simple sugars)

3. Iodine Solution, Lugol’s

Dissolve 20 g of potassium iodide in 200 mL of DI water; add 10 g of iodine; stir to dissolve then dilute to 1 L. Store in a dark bottle. (general biological stain and vital stain stock solution, dilute 5:1 before use.) Read more »